Isolation of a soluble tumor-associated antigen from human melanoma
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چکیده
منابع مشابه
Isolation of a soluble tumor-associated antigen from human renal cell carcinoma by gradient acrylamide gel electrophoresis.
of human tumors (1-10, 12-16). Since these extracts un doubtedly contain a spectrum of antigens, normal as well as tumor related, the isolation and purification of the TAA's would facilitate greatly the development of sensitive and specific immunological assays for the diagnosis and man agement of the cancer patient. Attempts to isolate the TAA's with the use of a variety of fractionation proce...
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Soluble extracts of melanoma specimens were prepared by 3 M KCl extraction. Delayed cutaneous hypersensitivity reactions to these antigens were noted in 25 of 39 melanoma patients and 7 of 30 patients with other neoplasms. Only 4 of 34 melanoma patients reacted to an extract of autologous muscle. Maximum reactivity to these antigens occurred at 24 hr and was demonstrated histologically by skin ...
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Cell surface melanoma-associated antigens can mediate cell-cell or cell-substrate adhesion, signal transduction, proteolysis, or immune recognition and play a key role in determining invasive and metastatic competence of the tumor cells. The melanoma-associated antigen, A32, was defined by a murine monoclonal antibody and was immunoprecipitated as a single 113 kDa integral membrane glycoprotein...
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The biosynthesis, structure, and topology of a melanoma-associated antigen, previously defined with the monoclonal antibody NKI/C-3 was studied. A polyclonal rabbit antiserum was raised against the antigen with a broader reactivity than the previously used monoclonal antibody NKI/C-3. The antigen was shown to consist of a single protein backbone to which two or three N-linked glycans were added...
متن کاملInhibition of leukocyte migration by tumor-associated antigen in soluble extracts of human hepatoma.
The leukocyte migration inhibition assay was used to detect cell-mediated immune reaction of hepatoma patients. Extraction of 3 pairs of human hepatoma tissue and normal liver tissue was made by 3 M KCI solution, and soluble hepatoma antigens were used to test against leukocytes of 18 hepatoma patients by the leukocyte migration inhibition assay. In all, 25 tests were performed; most of them we...
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ژورنال
عنوان ژورنال: Cancer
سال: 1976
ISSN: 0008-543X,1097-0142
DOI: 10.1002/1097-0142(197601)37:1<104::aid-cncr2820370115>3.0.co;2-i